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Cell-free tumour DNA : ウィキペディア英語版 | Cell-free tumour DNA
Cell-free or circulating tumour DNA (ctDNA) is tumour DNA circulating freely in the blood of a cancer patient. Analysis of the fraction of mutant-alleles from ctDNA compared to normal-alleles from the patients normal genome provides opportunities for minimally-invasive cancer diagnosis, prognosis and tumour monitoring. Cell-free DNA was first used medically for Down's syndrome screening using cell-free foetal DNA. ctDNA originates from tumour cells and can be present in a wide range of cancers but at varying levels and mutant allele fractions. The ctDNA is highly fragmented to around 170bp and is cleared rapidly after surgery to remove tumours or chemotherapeutic treatment. Protocols to extract ctDNA generally aim to reduce contamination with normal DNA from leukocytes. This is achieved by rapid processing of whole blood by centrifugation to remove all cells, and analysis of the remaining plasma. ==References==
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Cell-free tumour DNA」の詳細全文を読む
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